LOCUS       sequence-153435-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses smURFP and Heme Oxygenase-1 in bacterial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pBAD-smURFP-RBS-HO-1
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             497..514
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacgcttttt atcgcaactc tctactgttt ctcgataccc gtttttttgg gctagaaata
       61 attttgttta actttaagaa ggagatatgg atccatgaaa acttctgaac aacgtgtaaa
      121 catcgcaact ctgctgactg agaacaaaaa gaaaatcgtt gataaggcgt ctcaggatct
      181 gtggcgtcgt catccagatc tcattgcgcc gggcggcatc gcttttagtc aacgcgatcg
      241 tgcactgtgc ctgcgtgact acggctggtt tctgcacctg atcacgttct gtctgctggc
      301 tggtgataaa ggtcctattg aatccatcgg tctaattagc atacgcgaaa tgtacaacag
      361 cctgggtgtg ccggttccgg ctatgatgga atctatccgt tgtctgaaag aggcctccct
      421 gtcactgctg gacgaggaag acgccaatga gactgcaccg tactttgact atatcatcaa
      481 ggctatgtcc gaattccatc atcaccatca ccattgaaag aaggagatat acatacccat
      541 gagtgtcaac ttagcttccc agttgcggga agggacgaaa aaatcccact ccatggcgga
      601 gaacgtcggc tttgtcaaat gcttcctcaa gggcgttgtc gagaaaaatt cctaccgtaa
      661 gctggttggc aatctctact ttgtctacag tgccatggaa gaggaaatgg caaaatttaa
      721 ggaccatccc atcctcagcc acatttactt ccccgaactc aaccgcaaac aaagcctaga
      781 gcaagacctg caattctatt acggctccaa ctggcggcaa gaagtgaaaa tttctgccgc
      841 tggccaagcc tatgtggacc gagtccggca agtggccgct acggcccctc
//
