LOCUS       sequence-153436-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses smURFP and Heme Oxygenase-1 in bacterial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pBAD-smURFP-RBS-HO-1
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(771..788)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccaagctgg agaccgttta aacctagcct tcggaggtgg cgaggccaac ttcggtgctg
       61 ccttgactgc ggcgacgggt gaggctgttg aacaccataa tgccgatcgc cttgatcagg
      121 ttgccttcaa gttcgttgaa cattttcatg ttcatggcaa aggcgtcatt ggcttcatcc
      181 acaatccgtt cggcggtggc ttggtcaatg ggcagatcat tcatagcttg acggtaggta
      241 tttttaaaag ccttttcgtc atcaatgtcg gcaaattcat agaaagctgt gccaccatcg
      301 tggagattca tggcattttg ggcaattttc ttgagaattt gaccgccgga aagatccccc
      361 aggtaacggg tgtaggaatg ggccaccaac aattcagggg ccgtagcggc cacttgccgg
      421 actcggtcca cataggcttg gccagcggca gaaattttca cttcttgccg ccagttggag
      481 ccgtaataga attgcaggtc ttgctctagg ctttgtttgc ggttgagttc ggggaagtaa
      541 atgtggctga ggatgggatg gtccttaaat tttgccattt cctcttccat ggcactgtag
      601 acaaagtaga gattgccaac cagcttacgg taggaatttt tctcgacaac gcccttgagg
      661 aagcatttga caaagccgac gttctccgcc atggagtggg attttttcgt cccttcccgc
      721 aactgggaag ctaagttgac actcatgggt atgtatatct ccttctttca atggtgatgg
      781 tgatgatgga attcggacat agccttgatg atatagtcaa agtacggtgc agtctcattg
      841 gcgtcttcct cgtccagcag tgacagggag gcctctttca gacaacggat agattccatc
      901 atagccggaa ccggcacacc caggctgttg tacatttcgc gtatgctaat tagaccgatg
      961 gattcaatag gacc
//
