LOCUS       sequence-153439-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP77
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(69..102)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    780..827
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgaga
       61 tcagatctga agttcctata ctttctagag aataggaact tcaagcttca cttgcgctgg
      121 agacagtgcg atagggagct ctatggttcg agctaaccaa gttgtggcac tacaacaggg
      181 caatttcaga ctagtctgct gatttataat agaccctatg aaacagttat aacgccgcca
      241 agaattacta gattcccagt cctaaacatt acgtcatagt caaacgtatc caattgcgta
      301 cattgaatat aaccgaactt gagcaaaaac tgatgaataa cacctcaaga agtcaccccc
      361 tccgcgaacg attactaaac ttacgatata tggtgtgttt gtcatatgca attgtcacgt
      421 gaattgtgaa atagggccca atattcaagc agcttgaacc acgacgttct agagttccag
      481 cccttcttga aattgaagac agcgaccgaa aattgatcta cctaggaagg atacaccagg
      541 tgagtagcct ctgagacgta catatttttg tgcgtctgag tctttgcgtt catgagatta
      601 ttggaaacca ccagaatcga atataaaagg cgaacacctt tcccaatttt ggtttctcct
      661 gacccaaaga ctttaaattt aatttatttg tccctatttc aatcaattga acaactattt
      721 cggctggacg gcgacgtaaa cggccacaag tttatggccg tgatgacctg tgtcttcgtg
      781 gtttgtctgg tcaaccaccg cggtctcagt ggtgtacggt acaaacccaa agcagcacga
      841 cacggcaact acaagacccg cgccgagggc atgttc
//
