LOCUS       sequence-153440-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP77
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..102
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    780..813
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ttggggaaca tgccctcggc gcgggtcttg tagttgccgt gtcgtgctgc tttgggtttg
       61 taccgtacac cactgagacc gcggtggttg accagacaaa ccacgaagac acaggtcatc
      121 acggccataa acttgtggcc gtttacgtcg ccgtccagcc gaaatagttg ttcaattgat
      181 tgaaataggg acaaataaat taaatttaaa gtctttgggt caggagaaac caaaattggg
      241 aaaggtgttc gccttttata ttcgattctg gtggtttcca ataatctcat gaacgcaaag
      301 actcagacgc acaaaaatat gtacgtctca gaggctactc acctggtgta tccttcctag
      361 gtagatcaat tttcggtcgc tgtcttcaat ttcaagaagg gctggaactc tagaacgtcg
      421 tggttcaagc tgcttgaata ttgggcccta tttcacaatt cacgtgacaa ttgcatatga
      481 caaacacacc atatatcgta agtttagtaa tcgttcgcgg agggggtgac ttcttgaggt
      541 gttattcatc agtttttgct caagttcggt tatattcaat gtacgcaatt ggatacgttt
      601 gactatgacg taatgtttag gactgggaat ctagtaattc ttggcggcgt tataactgtt
      661 tcatagggtc tattataaat cagcagacta gtctgaaatt gccctgttgt agtgccacaa
      721 cttggttagc tcgaaccata gagctcccta tcgcactgtc tccagcgcaa gtgaagcttg
      781 aagttcctat tctctagaaa gtataggaac ttcagatctg atctcatgca tgacca
//
