LOCUS       sequence-153443-        5163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses smURFP and Heme Oxygenase-1 in bacterial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pBAD-smURFP-RBS-HO-1
FEATURES             Location/Qualifiers
     source          1..5163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             312..334
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             746..763
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1563..1580)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1733..1819
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1911..1938
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1958..2049
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2050..2118,2119..2910)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2268..2287)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3570..3589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3823..3840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3855..3995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3921..3940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4081..4103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4259..5137)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctgt
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagccg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tcgatacccg tttttttggg
      301 ctagaaataa ttttgtttaa ctttaagaag gagatatgga tccatgaaaa cttctgaaca
      361 acgtgtaaac atcgcaactc tgctgactga gaacaaaaag aaaatcgttg ataaggcgtc
      421 tcaggatctg tggcgtcgtc atccagatct cattgcgccg ggcggcatcg cttttagtca
      481 acgcgatcgt gcactgtgcc tgcgtgacta cggctggttt ctgcacctga tcacgttctg
      541 tctgctggct ggtgataaag gtcctattga atccatcggt ctaattagca tacgcgaaat
      601 gtacaacagc ctgggtgtgc cggttccggc tatgatggaa tctatccgtt gtctgaaaga
      661 ggcctccctg tcactgctgg acgaggaaga cgccaatgag actgcaccgt actttgacta
      721 tatcatcaag gctatgtccg aattccatca tcaccatcac cattgaaaga aggagatata
      781 catacccatg agtgtcaact tagcttccca gttgcgggaa gggacgaaaa aatcccactc
      841 catggcggag aacgtcggct ttgtcaaatg cttcctcaag ggcgttgtcg agaaaaattc
      901 ctaccgtaag ctggttggca atctctactt tgtctacagt gccatggaag aggaaatggc
      961 aaaatttaag gaccatccca tcctcagcca catttacttc cccgaactca accgcaaaca
     1021 aagcctagag caagacctgc aattctatta cggctccaac tggcggcaag aagtgaaaat
     1081 ttctgccgct ggccaagcct atgtggaccg agtccggcaa gtggccgcta cggcccctga
     1141 attgttggtg gcccattcct acacccgtta cctgggggat ctttccggcg gtcaaattct
     1201 caagaaaatt gcccaaaatg ccatgaatct ccacgatggt ggcacagctt tctatgaatt
     1261 tgccgacatt gatgacgaaa aggcttttaa aaatacctac cgtcaagcta tgaatgatct
     1321 gcccattgac caagccaccg ccgaacggat tgtggatgaa gccaatgacg cctttgccat
     1381 gaacatgaaa atgttcaacg aacttgaagg caacctgatc aaggcgatcg gcattatggt
     1441 gttcaacagc ctcacccgtc gccgcagtca aggcagcacc gaagttggcc tcgccacctc
     1501 cgaaggctag gtttaaacgg tctccagctt ggctgttttg gcggatgaga gaagattttc
     1561 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     1621 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     1681 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     1741 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1801 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     1861 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     1921 cctgacggat ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat
     1981 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2041 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2101 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2161 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2221 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     2281 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     2341 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2401 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2461 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2521 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2581 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2641 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2701 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2761 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2821 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2881 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2941 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3001 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3841 gaagcggtag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3901 cgtatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3961 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     4021 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4081 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga
     4141 tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag
     4201 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
     4261 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
     4321 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
     4381 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
     4441 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
     4501 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
     4561 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
     4621 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
     4681 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     4741 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     4801 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     4861 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     4921 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     4981 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     5041 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     5101 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
     5161 gag
//
