LOCUS       sequence-153448-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses smURFP and eGFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pcDNA3-smURFP-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..508
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgg ctaagacttc
      121 cgaacagagg gtgaacattg ctacactgct gacagaaaat aagaagaaaa tcgtggataa
      181 ggcttcccag gatctgtggc ggagacaccc agacctgatc gcaccaggag gaattgcttt
      241 ctctcagagg gaccgcgctc tgtgcctgcg agattacggc tggttcctgc atctgatcac
      301 cttttgtctg ctggccggag ataagggccc catcgagtct attgggctga tcagtattcg
      361 agaaatgtat aactcactgg gagtgcccgt ccctgcaatg atggagagca ttagatgcct
      421 gaaagaagcc agcctgtccc tgctggacga agaggacgcc aacgagaccg caccctactt
      481 tgattacatt attaaggcta tgagctaact cgagtactcc ggtattgcgg tacccttgta
      541 cgcctgtttt atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg
      601 ggtacaaacc agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac
      661 tgcttgcgtg gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt
      721 accacctcgg aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc
      781 tgatgagtct ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct
      841 atggctaacg ccatgggacg ctagttgtga acaaggtgtg aagagcct
//
