LOCUS       sequence-153453-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 7..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP69
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(30..63)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    673..720
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 ggattttggt catgcatgag atcagatctg aagttcctat actttctaga gaataggaac
       61 ttcaagcttc aacgatgcca aaagggtata ataagcgtca tttgcagcat tgtgaagaaa
      121 actatgtggc aagccaagcc tgcgaagaat gtattttaag tttgactttg atgtattcac
      181 ttgattaagc cataattctc gagtatctat gattggaagt atgggaatgg tgatacccgc
      241 attcttcagt gtcttgaggt ctcctatcag attatgccca actaaagcaa ccggaggagg
      301 agatttcatg gtaaatttct ctgacttttg gtcatcagta ggctcgaact gtgagactat
      361 ctcggttatg acagcagaaa tgtccttctt ggagacagta aatgaagtcc caccaataaa
      421 gaaatccttg tcatcaggaa caaacttctt gtttcgaact ttttcggtgc cttgaactat
      481 aatcatgaga ttattggaaa ccaccagaat cgaatataaa aggcgaacac ctttcccaat
      541 tttggtttct cctgacccaa agactttaaa tttaatttat ttgtccctat ttcaatcaat
      601 tgaacaacta tttcggctgg acggcgacgt aaacggccac aagtttatgg ccgtgatgac
      661 ctgtgtcttc gtggtttgtc tggtcaacca ccgcggtctc agtggtgtac ggtacaaacc
      721 caaagcagca cgacacggca actacaagac ccgcgccgag ggcatgttcc ccaaa
//
