LOCUS       sequence-153458-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculoviral entry vector to produce Bac-to-bac baculoviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pFastBac1-OSF-TRIM5alpha(rhesus)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..133
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             170..193
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             200..223
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             239..262
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg
       61 cggatcccgg tccgaagcgc gcggaattca aagggccacc atggctagct ggagccaccc
      121 gcagttcgag aaaggtggag gtgcccgagg tggatcggga ggtggatcgt ggagccaccc
      181 gcagttcgaa aaaggattcg attacaagga tgacgatgac aagggtacaa gatctccgct
      241 ggaagtcctg ttccagggcc ctatggcttc tggaatcctg cttaatgtaa aggaggaggt
      301 gacctgtccc atctgcctgg aactcctgac agaacccctg agtctgcact gcggccacag
      361 cttctgccaa gcgtgcatca ctgcgaacca caagaagtcc atgctataca aagaaggaga
      421 gagaagctgc cctgtgtgcc ggatcagtta ccagcctgag aacatacagc ctaatcggca
      481 tgtagccaac atagtggaga agctcaggga ggtcaagttg agcccagaag agggacagaa
      541 ggttgatcac tgtgcacgcc atggagagaa actcctactc ttctgtcagg aggacagcaa
      601 ggtcatttgc tggctttgtg agcggtctca ggagcaccgt ggtcaccaca ctttcctcat
      661 ggaggaggtt gcccaggagt accatgtgaa gctccagaca gctctggaga tgctgaggca
      721 gaagcagcag gaagctgaaa agttggaagc tgacatcaga gaagagaaag cttcctggaa
      781 gattcaaata gactacgaca aaaccaacgt ctcggcagat tttgagcaac tgagagagat
      841 cctg
//
