LOCUS       sequence-153459-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP67
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        84..560
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     protein_bind    622..669
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
       61 tggtcatgca tgagatcaga tcttttttgt agaaatgtct tggtgtcctc gtccaatcag
      121 gtagccatct ctgaaatatc tggctccgtt gcaactccga acgacctgct ggcaacgtaa
      181 aattctccgg ggtaaaactt aaatgtggag taatggaacc agaaacgtct cttcccttct
      241 ctctccttcc accgcccgtt accgtcccta ggaaatttta ctctgctgga gagcttcttc
      301 tacggccccc ttgcagcaat gctcttccca gcattacgtt gcgggtaaaa cggaggtcgt
      361 gtacccgacc tagcagccca gggatggaaa agtcccggcc gtcgctggca ataatagcgg
      421 gcggacgcat gtcatgagat tattggaaac caccagaatc gaatataaaa ggcgaacacc
      481 tttcccaatt ttggtttctc ctgacccaaa gactttaaat ttaatttatt tgtccctatt
      541 tcaatcaatt gaacaactat ttcggctgga cggcgacgta aacggccaca agtttatggc
      601 cgtgatgacc tgtgtcttcg tggtttgtct ggtcaaccac cgcggtctca gtggtgtacg
      661 gtacaaaccc aaagcagcac gacacggcaa ctacaagacc cgcgccgagg gcatgttccc
      721 caaagcgata ccacttgaag cagtggtact gcttgtgggt acactctgcg ggtgtgaagt
      781 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcttccaa ctcgcttaat
      841 tgcgagtt
//
