LOCUS       sequence-153460-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP67
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    103..150
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     promoter        complement(212..688)
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
ORIGIN
        1 ccgcagagtg tacccacaag cagtaccact gcttcaagtg gtatcgcttt ggggaacatg
       61 ccctcggcgc gggtcttgta gttgccgtgt cgtgctgctt tgggtttgta ccgtacacca
      121 ctgagaccgc ggtggttgac cagacaaacc acgaagacac aggtcatcac ggccataaac
      181 ttgtggccgt ttacgtcgcc gtccagccga aatagttgtt caattgattg aaatagggac
      241 aaataaatta aatttaaagt ctttgggtca ggagaaacca aaattgggaa aggtgttcgc
      301 cttttatatt cgattctggt ggtttccaat aatctcatga catgcgtccg cccgctatta
      361 ttgccagcga cggccgggac ttttccatcc ctgggctgct aggtcgggta cacgacctcc
      421 gttttacccg caacgtaatg ctgggaagag cattgctgca agggggccgt agaagaagct
      481 ctccagcaga gtaaaatttc ctagggacgg taacgggcgg tggaaggaga gagaagggaa
      541 gagacgtttc tggttccatt actccacatt taagttttac cccggagaat tttacgttgc
      601 cagcaggtcg ttcggagttg caacggagcc agatatttca gagatggcta cctgattgga
      661 cgaggacacc aagacatttc tacaaaaaag atctgatctc atgcatgacc aaaatccctt
      721 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
//
