LOCUS       sequence-153468-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miRFP PAS domain fused to FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pC4-RHE-PAS  (FRB-PAS)
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           9..48
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             495..773
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             780..806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctccggggga tcttggtggc gtgaaactcc cgcacctctt cggccagcga attccagaag
       61 ccgccaccat ggcttctaga atggtagcag gtcatgcctc tggcagcccc gcattcggga
      121 ccgcctctca ttcgaattgc gaacatgaag agatccacct cgccggctcg atccagccgc
      181 atggcgcgct tctggtcgtc agcgaacatg atcatcgcgt catccaggcc agcgccaacg
      241 ccgcggaatt tctgaatctc ggaagcgtac tcggcgttcc gctcgccgag atcgacggcg
      301 atctgttgat caagatcctg ccgcatctcg atcccaccgc cgaaggcatg ccggtcgcgg
      361 tgcgctgccg gatcggcaat ccctctacgg agtactgcgg tctgatgcat cggcctccgg
      421 aaggcgggct gatcatcgaa ctcgaacgtg ccggcccggg aggcggggga agcggcggag
      481 gcgggtcctc tagaatcctc tggcatgaga tgtggcatga aggcctggaa gaggcatctc
      541 gtttgtactt tggggaaagg aacgtgaaag gcatgtttga ggtgctggag cccttgcatg
      601 ctatgatgga acggggcccc cagactctga aggaaacatc ctttaatcag gcctatggtc
      661 gagatttaat ggaggcccaa gagtggtgca ggaagtacat gaaatcaggg aatgtcaagg
      721 acctcctcca agcctgggac ctctattatc atgtgttccg acgaatctca aagactagtt
      781 atccgtacga cgtaccagac tacgcataag aaaagtgagg atcctgagaa cttcagggtg
      841 agtttggg
//
