LOCUS       sequence-153469-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miRFP PAS domain fused to FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pC4-RHE-PAS  (FRB-PAS)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..79)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(86..364)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     regulatory      787..796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atcaagggtc cccaaactca ccctgaagtt ctcaggatcc tcacttttct tatgcgtagt
       61 ctggtacgtc gtacggataa ctagtctttg agattcgtcg gaacacatga taatagaggt
      121 cccaggcttg gaggaggtcc ttgacattcc ctgatttcat gtacttcctg caccactctt
      181 gggcctccat taaatctcga ccataggcct gattaaagga tgtttccttc agagtctggg
      241 ggccccgttc catcatagca tgcaagggct ccagcacctc aaacatgcct ttcacgttcc
      301 tttccccaaa gtacaaacga gatgcctctt ccaggccttc atgccacatc tcatgccaga
      361 ggattctaga ggacccgcct ccgccgcttc ccccgcctcc cgggccggca cgttcgagtt
      421 cgatgatcag cccgccttcc ggaggccgat gcatcagacc gcagtactcc gtagagggat
      481 tgccgatccg gcagcgcacc gcgaccggca tgccttcggc ggtgggatcg agatgcggca
      541 ggatcttgat caacagatcg ccgtcgatct cggcgagcgg aacgccgagt acgcttccga
      601 gattcagaaa ttccgcggcg ttggcgctgg cctggatgac gcgatgatca tgttcgctga
      661 cgaccagaag cgcgccatgc ggctggatcg agccggcgag gtggatctct tcatgttcgc
      721 aattcgaatg agaggcggtc ccgaatgcgg ggctgccaga ggcatgacct gctaccattc
      781 tagaagccat ggtggcggct tctggaattc gctggccgaa gaggtgcgg
//
