LOCUS       sequence-153477-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miRFP709 GAF domain fused to FKBP and NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pC4EN-F1-mGAF709  (NLS-FKBP-mGAF709)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           11..50
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             80..106
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             155..475
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gcctccgggg gatcttggtg gcgtgaaact cccgcacctc ttcggccagc gaattccaga
       61 agcgcgtatg gcttctagct atccttatga cgtgcctgac tatgccagcc tgggaggacc
      121 ttctagtcct aagaagaaga gaaaggtgtc tagaggagtg caggtggaaa ccatctcccc
      181 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat
      241 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat
      301 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
      361 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
      421 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactag
      481 tggaggcggg ggaagcggcg gaggcgggtc ccgtgccggc ccgtcgatcg atctgtcagg
      541 cacgctggcg ccggcgctgg agcggatccg cacggcgggt tcactgcgcg cgctgtgcga
      601 tgacaccgtg ctgctgtttc agcagtgcac cggctacgac cgggtgatgg tgtatcgttt
      661 cgatgagcaa ggccacggcc tggtattctc cgagtgccat gtgcctgggc tcgaatccta
      721 tttcggcaac cgctatccgt cgtcgttcat cccgcagatg gcgcggcagc tgtacgtgcg
      781 gcagcgcgtc cgcgtgctgg tcgacgtcac ctatcagccg gtgccgctgg agccgcggct
      841 gtcgccgctg acc
//
