LOCUS       sequence-153484-       14514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector containing doxycycline inducible human miR-200c. 
            Puromycin selectable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 14514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: pINDUCER13 miR-200c-141
FEATURES             Location/Qualifiers
     source          1..14514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    497..730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             915..959
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1257..1374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1439..1813)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1810..1819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1823..1841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1833..1880)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1950..1968,1969..1991,1992..2010,2011..2033,2034..2052,
                     2053..2075,2076..2094,2095..2117,2118..2136,2137..2159,
                     2160..2178,2179..2201,2202..2220)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2253..2291
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2288..2312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2382..2391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2388..4040
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2454..2472)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        4755..5966
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5803..5822
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5973..6006
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6013..6630,6631..6636,6637..6672,6673..6675,6676..6711,
                     6712..6714,6715..6750,6751..6759)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(6760..6793)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6809..7381
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6976..6993)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7203..7222
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7384..7986
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7883..7903
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8008..8596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8061..8081)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8479..8490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8803..9036
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9137..9154)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9143..9367
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9413..9841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9500..9519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9710..9731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9850..9870)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9855..10184
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10035..10170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10097..10116
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11007..11128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11177..11193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11177..11193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11190..11212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11201..11217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11225..11231,11232..11249,11250..11255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11270..11291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11408..11425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11579..12167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11659..11678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12338..13129,13130..13198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12961..12980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13199..13303)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13351..13485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             13653..14286
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ccgagtaata ccgggggaga ttagatcgcg atgggaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
      361 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
      421 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
      481 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
      541 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
      601 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
      661 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
      721 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
      781 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
      841 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
      901 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
      961 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1021 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1081 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1141 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     1201 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     1261 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1321 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1381 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gtccggaatc
     1441 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     1501 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     1561 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     1621 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     1681 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     1741 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     1801 tcaacttggc catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat
     1861 gccgatgatt aattgtcaac acgtgctgca ggtccgaggt tctaggctag cttaaaggaa
     1921 ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa agtgaaagtc
     1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2161 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2221 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2281 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2341 acctccatag aagacaccgg gaccgatcca gcctaccggt cgccaccatg gaagacgcca
     2401 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     2461 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     2521 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     2581 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     2641 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     2701 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     2761 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     2821 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     2881 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     2941 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     3001 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     3061 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     3121 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     3181 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     3241 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     3301 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     3361 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     3421 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     3481 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     3541 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     3601 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     3661 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     3721 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     3781 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     3841 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     3901 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     3961 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     4021 gcggaaagat cgccgtgtaa gcggccgcag ggctcaccag gaagtgtccc cagggactcg
     4081 ggtggtgggg ggatgggagc cagggatctg cagcttttcc gcagggatcc tgggcctgaa
     4141 gctgcctgac ccaaggtggg cgggctgggc gggggccctc gtcttaccca gcagtgtttg
     4201 ggtgcggttg ggagtctcta atactgccgg gtaatgatgg aggcccctgt ccctgtgtca
     4261 gcaacatcca tcgcctcagg tccccagccc ttagctggct gcagccccct ccccacttcc
     4321 cacgcacccc ggaagcccct cgtcttgagc tgagagcgtt gcacaagggg tggttcttgt
     4381 tggctggctg ccactaaggg acacaatggg ccccagcccc tcctcccacc cagtgcgatt
     4441 tgtcacctgg tggatccaga acccacagtc gaccttgagc ttggggttgg ctcgccccct
     4501 ctcaagagac ctcacctggc ctgtggccag ggtcccctgt agcaactggt gagcgcgcac
     4561 cgtagttctc tgtcggccgg ccctgggtcc atcttccagt acagtgttgg atggtctaat
     4621 tgtgaagctc ctaacactgt ctggtaaaga tggctcccgg gtgggttctc tcggcagtaa
     4681 ccttcaggga gccctgaaga ccatggagga ctactgacca acaacctctg accttcaccc
     4741 cacgcgtaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     4801 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
     4861 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
     4921 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
     4981 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
     5041 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
     5101 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
     5161 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
     5221 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
     5281 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
     5341 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
     5401 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
     5461 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     5521 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     5581 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     5641 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     5701 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     5761 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     5821 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     5881 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     5941 ggccgttttt ggcttttttg ttagacggat ccataacttc gtataatgta tgctatacga
     6001 agttatgcca ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta
     6061 ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     6121 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca
     6181 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     6241 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg
     6301 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     6361 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     6421 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag
     6481 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     6541 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     6601 ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac
     6661 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     6721 gctcttgacg attttgacct tgacatgctc cccgggtaaa taacttcgta tagcatacat
     6781 tatacgaagt tatggcgcgc caattccgcc cctctccctc ccccccccct aacgttactg
     6841 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     6901 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     6961 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     7021 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     7081 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     7141 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     7201 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     7261 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     7321 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
     7381 accatggcca ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     7441 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     7501 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     7561 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     7621 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg
     7681 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     7741 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     7801 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc
     7861 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7921 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7981 gcctgattaa ttaagcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     8041 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8101 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8161 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8221 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8281 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8341 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8401 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8461 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8521 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8581 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     8641 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     8701 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     8761 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8821 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8881 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8941 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9001 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9061 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgtt
     9121 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9181 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9241 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9301 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9361 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     9421 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9481 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9541 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9601 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9661 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9721 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9781 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9841 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9901 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10021 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10081 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    10141 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    10201 gtatatccat tttcggatct gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt
    10261 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
    10321 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
    10381 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
    10441 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
    10501 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
    10561 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
    10621 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
    10681 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
    10741 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
    10801 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
    10861 cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
    10921 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    10981 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    11041 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    11101 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    11161 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    11221 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    11281 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    11341 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    11401 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    11461 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11521 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11581 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11641 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11701 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11761 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11821 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11881 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11941 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    12001 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    12061 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    12121 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    12181 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    12241 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    12301 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    12361 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    12421 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    12481 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    12541 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    12601 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    12661 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    12721 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    12781 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    12841 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12901 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tccatacggg
    12961 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    13021 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    13081 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    13141 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    13201 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    13261 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    13321 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    13381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    13441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    13501 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    13561 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    13621 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
    13681 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
    13741 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
    13801 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
    13861 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
    13921 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
    13981 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
    14041 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
    14101 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    14161 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    14221 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    14281 ctagcagtgc cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
    14341 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaagggcggc gactggtgag
    14401 tacgccaaaa attttgacta gcggaaggct agaaggaaga agatggtgcc agagcgtcag
    14461 tattaagcgg gggaaaatta gatcgcgaag ggaaaaaatt cggttaaggc cagg
//
