LOCUS       sequence-153491-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses constitutively active WspR D70E under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pCOLA-T7-WspR:D70E
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    132..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             212..229
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        343..361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    362..386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       61 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga
      121 ctcactatag gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac
      181 tttaataagg agatatacca tgggcagcag ccatcaccat catcaccaca gccaggatcc
      241 gaattcgagc tcggcgcgcc tgcaggtcga caagcttgcg gccgcataat gcttaagtcg
      301 aacagaaagt aatcgtattg tacacggccg cataatcgaa attaatacga ctcactatag
      361 gggaattgtg agcggataac aattccccat cttagtatat tagttaagta taagaaggag
      421 atatacatat gcacaaccct catgagagca agaccgacct gggcgcccct ctggacggcg
      481 cggtcatggt actgcttgtc gatgatcagg ccatgatcgg agaggctgtg cgtcgctcac
      541 tggcgagcga ggcgggcatc gacttccatt tctgttccga tccgcagcag gcggtggcgg
      601 tggccaacca gatcaagccg acggtgatcc tccaggaact ggtgatgccc ggcgtcgacg
      661 gcctcacgct gctcgctgcc taccgcggca acccggcgac ccgcgacatc ccgatcatcg
      721 tcctgtcgac caaggaagag ccgacggtga agagcgcggc gttcgccgcc ggcgccaacg
      781 actacctggt caagctgccg gacgccatcg agctggtgg
//
