LOCUS       sequence-153492-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 
            (S/G2/M phase cell cycle reporter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pCSII-EF-miRFP670v1-hGem(1/110)
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..392)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgatc cctcgatgtt aactctagat
       61 tacagcgcct ttctccgttt ttctgccact tccttccaat actgagagga tggattttct
      121 ttaatcataa gatcaaatga ctcctgggtg actcctccaa gatttttatt ttcactagat
      181 tctgggacaa taaccccagg gctggaagtt gtagatgtta agtggtcatt ccgatgtttc
      241 cttttggaca agcctgcgga cagctcattt tctcttccaa caagagatcc agatgcagaa
      301 ggctgaatca tcttcagagt tcttcttggg acagaactat tctttatatt ctctttgatt
      361 tcttcttgtt tctgcttcat actgggattc atctcgagcg gccgcgatcc accacctcca
      421 gaaccacctc ctcctgatcc accacctcca gaacctcctc cacctgatcc accacctccg
      481 ctctcaagcg cggtgatccg cgtcgcgatc ctttcggcga gccgtttgca gatcgcccgc
      541 agctcgaaac ggatgaagcg cggcagataa tggtgacaga caaccaggcc ccacagcttg
      601 ccgccgacca ccagcgacac cgccagggtg gcgcgcacgc ccatgtcctt caggaactgc
      661 agatggcacg gcgacatcga gcgcaggaag cagcccgaca tgtcgagatc gcgcccggtc
      721 agcggcgaca gccgcggctc cagcggcacc ggctgatagg tgacgtcgac cagcacgcgg
      781 acgcgctgcc gcacgtacag ctgc
//
