LOCUS       sequence-153497-       10545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, 
            Klemm SL, van Oudenaarden A, Jaenisch R
  TITLE     Single-Cell Expression Analyses during Cellular Reprogramming Reveal
            an Early Stochastic and a Late Hierarchic Phase.
  JOURNAL   Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023.
  PUBMED    22980981
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1209-22"
COMMENT     Sequence Label: FUW-TetO-Ctcf
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2383..2401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2425..2443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2467..2485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2509..2527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2551..2569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2593..2611
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2644..2682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2679..2703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5078..5666
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5131..5151)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5549..5560)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5669..5685)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5670..5686)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6191..6371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6397..6414)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6403..6627
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6673..7101
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6760..6779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6970..6991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7110..7130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7154..7364
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7176..7372
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7223..7358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7285..7304
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7420..7467
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7486..7860
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7990..8111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8027..8046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8081..8100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8160..8176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8160..8176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8173..8195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8184..8200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8208..8214,8215..8232,8233..8238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8253..8274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8391..8408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8562..9150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8642..8661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9321..10112,10113..10181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9944..9963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10182..10286)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10361..10380)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
     2641 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     2701 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     2761 agcctccgcg gccccgaatt cggcttagag gaaggggaga tggaaggtga ggcggttgaa
     2821 gccattgtgg aggagtctga aactttcatt aaaggaaaag aaagaaagac ttaccagaga
     2881 cgccgggaag ggggccagga agaagatgct tgccacctgc cccagaacca gacagatggg
     2941 ggtgaggtgg tccaggatgt caacagcagt gtacagatgg taatgatgga acagctggat
     3001 cctacccttc tccagatgaa gactgaagtc atggagggta cagtggctcc ggaagcagag
     3061 gctgcagtgg acgataccca gatcataacc ttgcaggttg taaatatgga ggaacagccc
     3121 attaacatag gagagcttca gcttgtccaa gtacctgttc ctgtgacggt acctgttgct
     3181 actacttcag tagaagaact tcagggggct tatgagaatg aagtgtctaa agagggcctt
     3241 gcagaaagtg aaccgatgat atgtcacacc ttacctttgc ctgaaggatt tcaggtggtg
     3301 aaagtggggg ccaatggaga agtggagaca ctagagcagg gcgagcttcc tcctcaggaa
     3361 gactctagct ggcaaaaaga cccagactat cagccaccag ccaaaaaaac aaagaaaacc
     3421 aaaaagagca aacttcgtta cacagaagag ggcaaagacg tggatgtgtc tgtgtatgat
     3481 tttgaggaag aacagcagga aggactgctg tctgaggtta atgcagagaa agtagttggt
     3541 aatatgaagc ctccgaagcc aacaaaaatt aaaaaaaaag gtgtaaagaa aacattccag
     3601 tgtgagcttt gcagttacac atgtccccgg cgttcaaatt tggatcgtca catgaaaagc
     3661 cacactgatg agagaccaca caaatgccat ctgtgtggca gagcattcag aacagtgacc
     3721 ctcctgagga atcatctgaa cacacacaca ggtactcgtc ctcacaagtg cccagactgc
     3781 gatatggcct ttgtgaccag tggagaattg gtgcggcatc gtcgttataa acacactcat
     3841 gagaaaccat ttaagtgttc catgtgtgat tatgccagtg tagaagtcag caaattaaaa
     3901 cgacacattc gctctcatac tggagagcgc ccgttccagt gcagtttgtg cagttatgcc
     3961 agcagggaca catacaagct gaaaaggcat atgagaaccc attcagggga aaaaccttat
     4021 gaatgttata tttgtcacgc tcggtttacc cagagtggta ccatgaagat gcacatttta
     4081 cagaagcaca cagaaaatgt ggccaaattt cattgtcccc attgtgacac tgtcatagcc
     4141 cgaaaaagtg atttgggtgt ccacttgcga aagcagcatt cctatattga acagggcaaa
     4201 aaatgtcgct actgtgatgc tgtgtttcat gagcgatatg ctctcatcca gcatcaaaaa
     4261 tcacacaaaa atgagaagcg cttcaagtgt gaccagtgtg attatgcttg tagacaggag
     4321 cggcacatga tcatgcacaa gcgcactcac acgggggaga agccttatgc ctgcagccac
     4381 tgcgacaaga ccttccgcca gaaacagctc ctcgacatgc atttcaagcg ctatcatgat
     4441 cccaactttg tccctgctgc ctttgtctgt tccaagtgtg ggaaaacatt cacccgccgg
     4501 aacacaatgg caagacatgc agataactgt gctggtccag atggcgtaga gggggaaaat
     4561 ggaggggaga caaagaagag caaacgagga agaaaaagaa agatgcgatc taaaaaagaa
     4621 gactcctctg acagtgaaga aaatgctgag ccggatctcg atgacaatga ggaggaggag
     4681 gagcctgctg tagaaattga acctgagcca gagcctcagc cccagcctcc gcctccacct
     4741 cagcctgtgg ccccggcccc cccacctgcc aagaagagaa gggggcgacc tcctggaaga
     4801 accaaccagc ccaaacagaa ccagccaaca gccatcattc aggtcgaaga tcagaataca
     4861 ggtgcaattg agaacattat agttgaagtc aaaaaggagc cagatgccga gcctgcggag
     4921 ggggaagaag aggaggctca ggcagccacc acagacgccc ccaacggaga cctcacgcct
     4981 gagatgatcc tcagcatgat ggaccggtga tgctggggcc ttgctcggca ccaggactat
     5041 tgggctgtaa gccgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     5101 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5161 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5221 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5281 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5341 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5401 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5461 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5521 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5581 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5641 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     5701 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5761 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5881 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5941 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     6001 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     6061 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     6121 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     6181 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6241 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6301 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6361 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6421 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6481 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6541 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6601 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6661 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6961 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7021 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7081 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7201 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7321 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7381 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     7441 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7501 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7561 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7621 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7681 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7741 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7801 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7861 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7921 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7981 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8041 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8101 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8161 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8221 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8461 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8521 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8581 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8641 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8701 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8761 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8821 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8881 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8941 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9001 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9061 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9121 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9181 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9301 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9361 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9421 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9481 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9541 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9601 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9661 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9721 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9781 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9841 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9901 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9961 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10021 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10081 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10141 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10201 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10261 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    10321 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    10381 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10441 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10501 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atata
//
