LOCUS       sequence-153498-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector under the CMV promoter that expresses 
            smURFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pLenti-smURFP
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..463)
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
     regulatory      460..469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(488..507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(489..507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(552..755)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(581..601)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
       61 cttagctcat agccttaata atgtaatcaa agtagggtgc ggtctcgttg gcgtcctctt
      121 cgtccagcag ggacaggctg gcttctttca ggcatctaat gctctccatc attgcaggga
      181 cgggcactcc cagtgagtta tacatttctc gaatactgat cagcccaata gactcgatgg
      241 ggcccttatc tccggccagc agacaaaagg tgatcagatg caggaaccag ccgtaatctc
      301 gcaggcacag agcgcggtcc ctctgagaga aagcaattcc tcctggtgcg atcaggtctg
      361 ggtgtctccg ccacagatcc tgggaagcct tatccacgat tttcttctta ttttctgtca
      421 gcagtgtagc aatgttcacc ctctgttcgg aagtcttagc catggtggcg gatcccagct
      481 tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta
      541 gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      601 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      661 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
      721 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
      781 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
      841 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
      901 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
      961 attggcgtta ctatgggaac atacgtcatt attgacgtca atgg
//
