LOCUS       sequence-153501-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta A3-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBA3
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattactg ttggattcgg
      121 cgaatcgatt ggatctgcga agtctgggca tgagagcccc actctctcca atgtttatag
      181 tctcctccat gatggtcaca ttccaagata tactgatacc cacgatatcc aggatattgg
      241 tagcaaaccc aggccccact ttgtatcttc atggagccga cttcgttgtt gaaccagccc
      301 atggcttgca aggaggggta gtcgtcagag atctcccact ggcgtccaat aaagttttcc
      361 ttctcaaaga tggtcatctt agactcctta tgattagctg aacagatggg gcggaaggac
      421 atgagacgct caatgtggta ggcattactc ccactccagg catcccagcg agggtattct
      481 cctctctcca ggataaactg ttgcccacag aagctggtat gctcataacc aatccaggcg
      541 ccactttcca ccttcaggga ccggacatta tcaaaactgc gctcagagac atttggacag
      601 gagctggtga actccatcct cttgccctga aagttctcct gatcatagat ggttatcttc
      661 catggcccca gggaccccgg cgtagggttg gtctgagcca tcttggtggt tggaagggtt
      721 tccagctcct gctgctcagc ctgggtctcc atacctccaa tctgttcgcg gtgagcctca
      781 ataatatcgt tatcctccat gtccaaatct tcaggggcct gatcagcttg aattctaata
      841 ccgtcgtaca agaatcttaa ggagtccatt tccttaccct gtcttttagc gaacgcttcc
      901 atcagccttc ttaaaggagt ggtctttttg atcttgaaga agatctctga agatccatcg
      961 g
//
