LOCUS       sequence-153505-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses under the CAG promoter GFE3, a synthetic E3 ligase that 
            rapidly degrades endogenous Gephyrin at inhibitory synapses..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts 
            RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, 
            Arnold DB
  TITLE     An E3-ligase-based method for ablating inhibitory synapses.
  JOURNAL   Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.
  PUBMED    27271196
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894."
COMMENT     Sequence Label: pCAG_EGFP-GFE3
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(30..49)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          32..164
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(384..464)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgaca cctgtggaga gaaaggcaaa gtggatgtca
       61 gtaagaccaa taggtgccta tcagaaacgc aagagtcttc tctgtctcga caagcccagt
      121 ttctattggt ctccttaaac ctgtcttgta accttgatac ttacctgcgg cctcgagtcc
      181 ggggccgctt tacttgtaca ttattaagac ataaaaattt tttgcttgaa cgtaatgact
      241 gtgcagcaca tgggacattt gtcaactgct tccgcacact gtttacaagt gaccagatgt
      301 ccacaaggaa caaaaactat agcaatattt ctatccatac agattttgca aagcttctcc
      361 tcttgtaggc gccttaggct agcagcgtaa tctggaacat cgtatgggta agcgtaatct
      421 ggaacatcgt atgggtaagc gtaatctgga acatcgtatg ggtaagatct tgaaccggtg
      481 cggtagttga tggagatcgg gatcgggtcc tggtagttcc agtggtcgat cgtgacggcg
      541 tacaccgtga tggtatagtc gacaccaggt ttcaggctgc tgatggtagc agtggacttg
      601 ctgccaggca cggtgaattc ctggacaggg ctattgccac cagtttcacc gtaggtgatg
      661 cggtagtagc gaaccatgac cttgtacttg ccccagctga tctggatgct ggttggtgat
      721 gcttccttga cttcgagcaa ttgcgaacct cctcctgatc cacctccaga acctccacca
      781 gaacctccac cgtacagctc gtc
//
