LOCUS       sequence-153511-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta B2-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBB2
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctctaga ttagttggag gggtggaagg
      121 caccacgttg gtgccactgc atgtcgcgga tacggcgcac ggactgcacc tgggggtgag
      181 gggccccaaa gtcgctgctg tccttgtagt ctcccttctc cagcaggtac tgcagtccac
      241 ggtagccggg gtactggtag ccaacccacg tgccactctg cacccgcaca gatgacacct
      301 tctcctggta gccatgggcg tggaagctgg gtacatcgtc atctatgatt tccatcttct
      361 tcccggtgaa gttggggttt tcatagagga tgatcttgtg ctcttggctg tccactttga
      421 tgggcctcag ggagctgagg gagtccgtcc ttcggctgct ggtccatgag tcccagcggg
      481 ggtactcacc cttctcaaac acaaactgct cgcccttgca gttggcctgt tcatagccca
      541 cccagggtcc agcctgcact aggacagaac ctgccttctc cacgccagtt tccttcaggt
      601 tggggcaggg cccattgagc tcatgcgagt ggccttgaaa gttttcctgc tcaaagatga
      661 tgatcttggg gttgagggac tgtggcttgc ccgcctgggt ctggtgatct gaggcacctc
      721 caatctgttc gcggtgagcc tcaataatat cgttatcctc catgtccaaa tcttcagggg
      781 cctgatcagc ttgaattcta ataccgtcgt acaagaatct taaggagtcc atttccttac
      841 cctgtctttt agcgaacgct tccatcagcc ttcttaaagg agtggtcttt ttgatcttga
      901 agaagatctc tgaagatcca tcggacacct ttaaattgat gtgagtctca ggcttgact
//
