LOCUS       sequence-153515-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP1-10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHR-SFFV-GFP1-10
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..727)
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat caagcttgca tgcctgcagg
       61 tcgactctag agtcgcggcc gcttattttt catttggatc tttgctcagg actgtttgtg
      121 ttgagaggta gtgattatca ggaagaagta ccggaccgtc tccaataggg gtgttttgtt
      181 ggtaatggtc ggcaagttga acactgccat cttctacatt atggcgtact gtgaaattcg
      241 ctttaattcc gttcttttgc ttatcagcag tgatgtatac gttatgacta ttaaaattgt
      301 attcgagttt gtgtccaaga atattaccat cttctttaaa atctgttcct ttcagttcaa
      361 tgcggttaac caaagtatct ccttcaaatt taacaactgc tctagtttta tattttccat
      421 catctttgaa cgaaattgtt ctttcttgca cgtaaccttc aggcatagcc gacttaaaaa
      481 agtcatgtct tttcatatgg tctgggtatc tcgaaaagca ctgaactccg tacgtaagcg
      541 ttgttacgag agtcggccat ggtacaggaa gtttaccagt agtacagatg aatttaagag
      601 tcaattttcc aatggtggcg tcaccttctc cttcgcctct cactgagaac ttatgtccgt
      661 tgacatcacc atcgagttca accaaaattg gcacaacacc ggtaaacagt tcttctcctt
      721 tggacatggt ggcggcggat cccgggcccg cggtaccgca agcttgatat cctgcagacg
      781 cgtgagaatt ctcgagagct ccagatccc
//
