LOCUS       sequence-153517-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta B3-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBB3
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..371
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggtcatcacc
       61 atcatcatca cgggtccctg caggactcag aagtcaatca agaagctaag ccagaggtca
      121 agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga
      181 tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa
      241 gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg
      301 atcaggcccc tgaagatttg gacatggagg ataacgatat tattgaggct caccgcgaac
      361 agattggagg tatggcggaa cagcacggag cacccgaaca ggctgcagct ggcaagagcc
      421 atggagacct tgggggcagc tacaaggtga tcttgtacga actagagaac ttccaaggca
      481 aacgctgcga gctctcggcc gagtgcccca gcctgaccga cagcctgctg gagaaggtgg
      541 gctccatcca agtggagtcc gggccgtggc tggcatttga gtccagggcc ttccgcgggg
      601 agcagtttgt tctggagaag ggggattatc ctcgctggga tgcctggtcc aacagccgtg
      661 atagtgacag ccttctgtcc ctccggcctc tgaatattga tagtccagat cacaagctgc
      721 atctgtttga gaacccagct ttcagtggcc gcaagatgga gatagtggat gatgacgtgc
      781 ccagcctgtg ggctcatggc ttccaggacc gtgtggcgag tgtccgtgcc atcaacggga
      841 cgtgggttgg ctatgagttc cccggctacc gtgggcgcca gtacgtgttt gagcg
//
