LOCUS       sequence-153518-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta B3-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBB3
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattagct gctggggaag
      121 cggccccgct tgtgccactt ctggtcacgg atgcggcgca cagactgcag ctgcggctgg
      181 ctggcgtccc actcattcca gtggcggtac tcgccccgct caaacacgta ctggcgccca
      241 cggtagccgg ggaactcata gccaacccac gtcccgttga tggcacggac actcgccaca
      301 cggtcctgga agccatgagc ccacaggctg ggcacgtcat catccactat ctccatcttg
      361 cggccactga aagctgggtt ctcaaacaga tgcagcttgt gatctggact atcaatattc
      421 agaggccgga gggacagaag gctgtcacta tcacggctgt tggaccaggc atcccagcga
      481 ggataatccc ccttctccag aacaaactgc tccccgcgga aggccctgga ctcaaatgcc
      541 agccacggcc cggactccac ttggatggag cccaccttct ccagcaggct gtcggtcagg
      601 ctggggcact cggccgagag ctcgcagcgt ttgccttgga agttctctag ttcgtacaag
      661 atcaccttgt agctgccccc aaggtctcca tggctcttgc cagctgcagc ctgttcgggt
      721 gctccgtgct gttccgccat acctccaatc tgttcgcggt gagcctcaat aatatcgtta
      781 tcctccatgt ccaaatcttc aggggcctga tcagcttgaa ttctaatacc gtcgtacaag
      841 aatcttaagg agtccatttc cttaccctgt cttttagcga acgcttccat cagccttctt
      901 aaaggagtgg tctttttgat cttgaagaag atctctgaag atccatcgga cacctttaaa
      961 ttgatgtgag tctcaggctt
//
