LOCUS       sequence-153520-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma B-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGB
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..365
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     CDS             943..960
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggtcat caccatcatc
       61 atcacgggtc cctgcaggac tcagaagtca atcaagaagc taagccagag gtcaagccag
      121 aagtcaagcc tgagactcac atcaatttaa aggtgtccga tggatcttca gagatcttct
      181 tcaagatcaa aaagaccact cctttaagaa ggctgatgga agcgttcgct aaaagacagg
      241 gtaaggaaat ggactcctta agattcttgt acgacggtat tagaattcaa gctgatcagg
      301 cccctgaaga tttggacatg gaggataacg atattattga ggctcaccgc gaacagattg
      361 gaggtggaaa gatcaccttc tacgaggaca gggccttcca gggccgcagc tacgaatgca
      421 ccactgactg ccccaaccta caaccctatt tcagccgctg caactccatc agggtggaga
      481 gcggctgctg gatgatctat gagcgcccca actaccaggg ccaccagtac ttcctgcggc
      541 gtggggagta ccccgactac cagcaatgga tgggcctcag cgactccatc cgctcctgct
      601 gcctcatccc cccgcactct ggcgcttaca gaatgaagat ctacgacaga gatgaattga
      661 ggggacaaat gtcagagctc acagacgact gtctctctgt tcaggaccgc ttccacctca
      721 ctgaaattca ctccctcaat gtgctggagg gcagctggat cctctatgag atgcccaact
      781 acagggggag gcagtatctg ctgaggccgg gggagtacag gaggtttctt gattgggggg
      841 ctccaaatgc caaagttggc tctcttagac gagtcatgga tttgtactaa tctagaggat
      901 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      961 tgagat
//
