LOCUS       sequence-153521-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma B-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGB
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(630..917)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(930..947)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             960..982
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattagta caaatccatg
      121 actcgtctaa gagagccaac tttggcattt ggagcccccc aatcaagaaa cctcctgtac
      181 tcccccggcc tcagcagata ctgcctcccc ctgtagttgg gcatctcata gaggatccag
      241 ctgccctcca gcacattgag ggagtgaatt tcagtgaggt ggaagcggtc ctgaacagag
      301 agacagtcgt ctgtgagctc tgacatttgt cccctcaatt catctctgtc gtagatcttc
      361 attctgtaag cgccagagtg cggggggatg aggcagcagg agcggatgga gtcgctgagg
      421 cccatccatt gctggtagtc ggggtactcc ccacgccgca ggaagtactg gtggccctgg
      481 tagttggggc gctcatagat catccagcag ccgctctcca ccctgatgga gttgcagcgg
      541 ctgaaatagg gttgtaggtt ggggcagtca gtggtgcatt cgtagctgcg gccctggaag
      601 gccctgtcct cgtagaaggt gatctttcca cctccaatct gttcgcggtg agcctcaata
      661 atatcgttat cctccatgtc caaatcttca ggggcctgat cagcttgaat tctaataccg
      721 tcgtacaaga atcttaagga gtccatttcc ttaccctgtc ttttagcgaa cgcttccatc
      781 agccttctta aaggagtggt ctttttgatc ttgaagaaga tctctgaaga tccatcggac
      841 acctttaaat tgatgtgagt ctcaggcttg acttctggct tgacctctgg cttagcttct
      901 tgattgactt ctgagtcctg cagggacccg tgatgatgat ggtgatgacc catggtatat
      961 ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg ct
//
