LOCUS       sequence-153526-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta A1-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBA1
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..371
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggtcatcacc
       61 atcatcatca cgggtccctg caggactcag aagtcaatca agaagctaag ccagaggtca
      121 agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga
      181 tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa
      241 gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg
      301 atcaggcccc tgaagatttg gacatggagg ataacgatat tattgaggct caccgcgaac
      361 agattggagg tgctcagacc aaccctacgc cggggtccct ggggccatgg aagataacca
      421 tctatgatca ggagaacttt cagggcaaga ggatggagtt caccagctcc tgtccaaatg
      481 tctctgagcg cagttttgat aatgtccggt ccctgaaggt ggaaagtggc gcctggattg
      541 gttatgagca taccagcttc tgtgggcaac agtttatcct ggagagagga gaataccctc
      601 gctgggatgc ctggagtggg agtaatgcct accacattga gcgtctcatg tccttccgcc
      661 ccatctgttc agctaatcat aaggagtcta agatgaccat ctttgagaag gaaaacttta
      721 ttggacgcca gtgggagatc tctgacgact acccctcctt gcaagccatg ggctggttca
      781 acaacgaagt cggctccatg aagatacaaa gtggggcctg ggtttgctac caatatcctg
      841 gatatcgtgg gtatcagtat atcttggaat gtgaccatca tggaggagac tataaacatt
      901 ggagagag
//
