LOCUS       sequence-153527-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta A1-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBA1
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(699..986)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattactg ttggattcgg
      121 cgaatcgatt ggatctgcga agtctgggca tgagagcccc actctctcca atgtttatag
      181 tctcctccat gatggtcaca ttccaagata tactgatacc cacgatatcc aggatattgg
      241 tagcaaaccc aggccccact ttgtatcttc atggagccga cttcgttgtt gaaccagccc
      301 atggcttgca aggaggggta gtcgtcagag atctcccact ggcgtccaat aaagttttcc
      361 ttctcaaaga tggtcatctt agactcctta tgattagctg aacagatggg gcggaaggac
      421 atgagacgct caatgtggta ggcattactc ccactccagg catcccagcg agggtattct
      481 cctctctcca ggataaactg ttgcccacag aagctggtat gctcataacc aatccaggcg
      541 ccactttcca ccttcaggga ccggacatta tcaaaactgc gctcagagac atttggacag
      601 gagctggtga actccatcct cttgccctga aagttctcct gatcatagat ggttatcttc
      661 catggcccca gggaccccgg cgtagggttg gtctgagcac ctccaatctg ttcgcggtga
      721 gcctcaataa tatcgttatc ctccatgtcc aaatcttcag gggcctgatc agcttgaatt
      781 ctaataccgt cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagcgaac
      841 gcttccatca gccttcttaa aggagtggtc tttttgatct tgaagaagat ctctgaagat
      901 ccatcggaca cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctggc
      961 ttagcttctt gattgacttc tgagtcctgc agggac
//
