LOCUS       sequence-153528-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta A2-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBA2
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..360
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tataccatgg gtcatcacca tcatcatcac
       61 gggtccctgc aggactcaga agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc
      121 aagcctgaga ctcacatcaa tttaaaggtg tccgatggat cttcagagat cttcttcaag
      181 atcaaaaaga ccactccttt aagaaggctg atggaagcgt tcgctaaaag acagggtaag
      241 gaaatggact ccttaagatt cttgtacgac ggtattagaa ttcaagctga tcaggcccct
      301 gaagatttgg acatggagga taacgatatt attgaggctc accgcgaaca gattggaggt
      361 agcagcgccc ccgcgccggg cccggcgccc gccagcctca cgctctggga cgaggaggac
      421 ttccagggcc gtcgctgtcg gctgctaagc gactgtgcga acgtctgcga gcgcggaggc
      481 ctgcccaggg tgcgctcggt caaggtggaa aacggcgttt gggtggcctt tgagtacccc
      541 gacttccagg gacagcagtt cattctggag aagggagact atcctcgctg gagcgcctgg
      601 agtggcagca gcagccacaa cagcaaccag ctgctgtcct tccggccagt gctctgcgcg
      661 aaccacaatg acagccgtgt gacactgttt gagggggaca acttccaagg ctgcaagttt
      721 gacctcgttg atgactaccc atccctgccc tccatgggct gggccagcaa ggatgtgggt
      781 tccctcaaag tcagctccgg agcgtgggtg gcctaccagt acccaggcta ccgaggctac
      841 cagtatgtgt tggagcggga ccggcacagc ggagagttct gtacttacgg tgag
//
