LOCUS       sequence-153529-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human beta A2-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYBA2
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(696..983)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattagtg ctggactctc
      121 cggatggact gcagctgccc agtgtgggcc tgtgtgccga gctcaccgta agtacagaac
      181 tctccgctgt gccggtcccg ctccaacaca tactggtagc ctcggtagcc tgggtactgg
      241 taggccaccc acgctccgga gctgactttg agggaaccca catccttgct ggcccagccc
      301 atggagggca gggatgggta gtcatcaacg aggtcaaact tgcagccttg gaagttgtcc
      361 ccctcaaaca gtgtcacacg gctgtcattg tggttcgcgc agagcactgg ccggaaggac
      421 agcagctggt tgctgttgtg gctgctgctg ccactccagg cgctccagcg aggatagtct
      481 cccttctcca gaatgaactg ctgtccctgg aagtcggggt actcaaaggc cacccaaacg
      541 ccgttttcca ccttgaccga gcgcaccctg ggcaggcctc cgcgctcgca gacgttcgca
      601 cagtcgctta gcagccgaca gcgacggccc tggaagtcct cctcgtccca gagcgtgagg
      661 ctggcgggcg ccgggcccgg cgcgggggcg ctgctacctc caatctgttc gcggtgagcc
      721 tcaataatat cgttatcctc catgtccaaa tcttcagggg cctgatcagc ttgaattcta
      781 ataccgtcgt acaagaatct taaggagtcc atttccttac cctgtctttt agcgaacgct
      841 tccatcagcc ttcttaaagg agtggtcttt ttgatcttga agaagatctc tgaagatcca
      901 tcggacacct ttaaattgat gtgagtctca ggcttgactt ctggcttgac ctctggctta
      961 gcttcttgat tgacttctga gtcctgcagg gacccgtgat ga
//
