LOCUS       sequence-153533-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE65
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..559)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(601..780)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ggggtcatca gatttcggtg acgggcagga ccggacgggg cggcaccggc aggctgaagt
       61 ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca
      121 tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc
      181 cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt
      241 agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg
      301 ccgtccagtc gtaggcgttg cgtgccttcc agggacccgc gtaggcgatg ccggcgacct
      361 cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg
      421 tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt
      481 ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg
      541 ggcgtcgttc tgggctcatg gtagatcccc tcgatcgagt tgagagtgaa tatgagactc
      601 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
      661 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
      721 tgcttagctc attaaactcc agaaacccgc ggctcagtgg ctccttcaac gttgcggttc
      781 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
      841 cccttaattc tccgctcatg attagtagcg gtcctgctga gcctcgacat gttgtcgcaa
      901 aattcgccct ggacccgccc aacgatttgt cgtcactgtc aaggtttgac ctgcacttca
      961 tttggggccc acatacacca aaaaaatgct gcataat
//
