LOCUS       sequence-153538-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE166
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        591..936
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     879..899
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctcg gtctctgcaa gaattcaagc
      181 ttggaggtca acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca
      241 gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc
      301 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt
      361 ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc
      421 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt
      481 ccaaccacgt ctacaaagca agtggattga tgtgataaca tggtggagca cgacactctg
      541 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      601 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      661 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      721 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      781 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      841 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
      901 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
      961 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
     1021 gatacaatgg acaagaagta ctccattggg ctcgatatcg gcacaaacag cgtcggctgg
     1081 gccgtcatta cggacgagta caagg
//
