LOCUS       sequence-153540-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; p35S:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE62
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     971..991
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca gaagacttca atcactactt
      181 cgactctagc tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg
      241 aagattgttt tcaacttagc aagaattcaa gcttggaggt caacatggtg gagcacgaca
      301 ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agatcaaagg gctattgaga
      361 cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
      421 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
      481 aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat ggacccccac
      541 ccacgaggag catcgtggaa aaagaagagg ttccaaccac gtctacaaag caagtggatt
      601 gatgtgataa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag
      661 tctcagaaga tcaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc
      721 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg
      781 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg
      841 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagaggttc
      901 caaccacgtc tacaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
      961 cacaatccca ctatccttcg caagaccctt cctc
//
