LOCUS       sequence-153541-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; p35S:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE62
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(63..246)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(99..278)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(371..623)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(639..659)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atccagatcc
       61 ggtgcagatt atttggattg agagtgaata tgagactcta attggatacc gaggggaatt
      121 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
      181 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
      241 aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
      301 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
      361 tagtagcgtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
      421 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
      481 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
      541 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
      601 tattgccaaa tgtttgaacg atctgcttga caagctcaca ccttcctctt cttcttgggg
      661 tcagccctgc tgtctccacc gagctgagag aggtcgattc ttgtttcata gagccccgta
      721 attgactgat gaatcagtgt ggcgtccagg acctcctttg tagaggtgta ccgctttctg
      781 tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac
      841 aagtggataa tgttttctgc ctgctccctg atgggcttat ccctgtgctt attgtaagca
      901 gaaagcacct tatcgaggtt agcgtcggcg aggatcactc ttttggagaa ttcgcttatt
      961 tgctcgatga
//
