LOCUS       sequence-153545-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP255
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(567..813)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 tgaattctgt gttttgatag ttgttcaatt gattgaaata gggacaaata aattaaattt
       61 aaagtctttg ggtcagggga aaccaaaatt gggaaaggtg ttcgcctttt atattcgatt
      121 ctggtggttt ccaataatct catgacatgc gtccgcccgc tattattgcc agcgacggcg
      181 cgcccatagg gtatcctcac tccgattgat gaagagaccc ttctggttcc aatcctcact
      241 ccgattgatg tgacgtcaat gggtggagta tatcctcact ccgattgatg agtgctctcg
      301 atgacttcga tatcctcact ccgattgatg aactccgccc cattgacgca aatcctcact
      361 ccgattgatg gtggaacagc ccaccctgta catcctcact ccgattgatg tgcggcctcg
      421 agaagcagct gaatcctcac tccgattgat gactggaccg tcaccaattg gagatcctca
      481 ctccgattga tggtggtaaa ctcgcaatat cctcactccg attgatgaat tgatctgccg
      541 cgatgacttc tatatgatat gctagctctc acttaatctt ctgtactctg aagaggagtg
      601 ggaaatacca agaaaaacat caaactcgaa tgattttccc aaacccctac cacaagatat
      661 tcatcagctg cgagataggc tgatcaggag caagctcgta cgagaagaaa caaaatgaca
      721 aaaaaaatcc tatactatat aggttacaaa taaaaaagta tcaaaaatga agcctgcatc
      781 tctcaggcaa atggcattct gacatcctct tgattagaat ctagcaagac cggtctaccg
      841 atggtacctc aagaagcgta gtccggaacg tcgtacg
//
