LOCUS       sequence-153548-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP. Figure 3. Strain 2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP296
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(579..825)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(867..893)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatgaattc gcgtttcgat ttaagcttcg aattgatccg gtaatttagt gtgtgtattt
       61 gtgtttgcgt gtctatagaa gtatagtaat ttatgctaca aaggacctaa tgtataagga
      121 aagaatattt agagaaaaga agaaaacaag agttttatat acatacagag cacatgcatg
      181 ccatagggtg gcgcgccata gggtatgaca ctctcctggt tccgattctc accccaattg
      241 aagtcacatc gattggagga gtgtaccctt attcagactg gtgagtggac tcgataactt
      301 ctatgtcctc ggatctgttg ataaattcgg ccccattcac acagattctg acaccgatag
      361 atgggggaac ggcccatccg gtgcaccctt actctgactg gtgagcagct tccagcaaca
      421 attgaatacg aactccgatt gaactttgaa cagtaaccaa ttgtaaatcc tcacttctat
      481 taatagtagt aaattcacaa tacccctatt ctgactgatg atcctcactc cgattgatga
      541 attgatctgc cgcgatgact tctatatgat atgctagctc tcacttaatc ttctgtactc
      601 tgaagaggag tgggaaatac caagaaaaac atcaaactcg aatgattttc ccaaacccct
      661 accacaagat attcatcagc tgcgagatag gctgatcagg agcaagctcg tacgagaaga
      721 aacaaaatga caaaaaaaat cctatactat ataggttaca aataaaaaag tatcaaaaat
      781 gaagcctgca tctctcaggc aaatggcatt ctgacatcct cttgattaga atctagcaag
      841 accggtctac cgatggtacc tcaagaagcg tagtccggaa cgtcgtacgg gtagttaatt
      901 aacatatcga gatcga
//
