LOCUS       sequence-153555-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP. Figure 3. Strain 3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP297
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(588..834)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(876..902)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccttgctcac catgaattcg cgtttcgatt taagcttcga attgatccgg taatttagtg
       61 tgtgtatttg tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat
      121 gtataaggaa agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc
      181 acatgcatgc catagggtgg cgcgccatag ggtatgacac tctcctggtt ccgattctca
      241 ccccaattga agtcacatcg attggaggag tgtaccctta ttcagactgg tgagtggact
      301 cgataacttc tatgtcctcg gatctgttga taaattcggc cccattcaca cagattctga
      361 caccgataga tgatcctcac tccgattgat gggggaacgg cccatccggt gcacccttac
      421 tctgactggt gagcagcttc cagcaacaat tgaatacgaa ctccgattga actttgaaca
      481 gtaaccaatt gtaaatcctc acttctatta atagtagtaa attcacaata cccctattct
      541 gactgatgaa ttgatctgcc gcgatgactt ctatatgata tgctagctct cacttaatct
      601 tctgtactct gaagaggagt gggaaatacc aagaaaaaca tcaaactcga atgattttcc
      661 caaaccccta ccacaagata ttcatcagct gcgagatagg ctgatcagga gcaagctcgt
      721 acgagaagaa acaaaatgac aaaaaaaatc ctatactata taggttacaa ataaaaaagt
      781 atcaaaaatg aagcctgcat ctctcaggca aatggcattc tgacatcctc ttgattagaa
      841 tctagcaaga ccggtctacc gatggtacct caagaagcgt agtccggaac gtcgtacggg
      901 tagttaatta acatatcgag atcga
//
