LOCUS       sequence-153558-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma S-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGS
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..364
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggtcatc accatcatca
       61 tcacgggtcc ctgcaggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga
      121 agtcaagcct gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt
      181 caagatcaaa aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg
      241 taaggaaatg gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcaggc
      301 ccctgaagat ttggacatgg aggataacga tattattgag gctcaccgcg aacagattgg
      361 aggttctaaa actggaacca agattacttt ctatgaagac aaaaattttc aaggccgtcg
      421 ctatgactgt gattgcgact gtgcagattt ccacacatac ctaagtcgct gcaactccat
      481 taaagtggaa ggaggcacct gggctgttta tgaaaggccc aactttgctg ggtacatgta
      541 catcttacca cagggagagt accctgaata ccagcgttgg atgggcctca acgaccgcct
      601 cagctcctgc agagctgttc atctgcctag tggaggccag tataagattc agatctttga
      661 gaaaggggat tttagtggtc agatgtatga aaccaccgaa gattgccctt ccatcatgga
      721 gcaatttcac atgcgagaga tccactcctg taaggtgctg gagggtgtct ggattttcta
      781 tgagctaccc aactaccgtg gcaggcagta cctcctggac aagaaggagt accggaagcc
      841 catcgattgg ggtgcagcct ccccagctgt ccagtctttc cgccgcattg tggagtaatc
      901 tagaggatcc gaat
//
