LOCUS       sequence-153559-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma S-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGS
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(639..926)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(939..956)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             969..991
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattactc cacaatgcgg
      121 cggaaagact ggacagctgg ggaggctgca ccccaatcga tgggcttccg gtactccttc
      181 ttgtccagga ggtactgcct gccacggtag ttgggtagct catagaaaat ccagacaccc
      241 tccagcacct tacaggagtg gatctctcgc atgtgaaatt gctccatgat ggaagggcaa
      301 tcttcggtgg tttcatacat ctgaccacta aaatcccctt tctcaaagat ctgaatctta
      361 tactggcctc cactaggcag atgaacagct ctgcaggagc tgaggcggtc gttgaggccc
      421 atccaacgct ggtattcagg gtactctccc tgtggtaaga tgtacatgta cccagcaaag
      481 ttgggccttt cataaacagc ccaggtgcct ccttccactt taatggagtt gcagcgactt
      541 aggtatgtgt ggaaatctgc acagtcgcaa tcacagtcat agcgacggcc ttgaaaattt
      601 ttgtcttcat agaaagtaat cttggttcca gttttagaac ctccaatctg ttcgcggtga
      661 gcctcaataa tatcgttatc ctccatgtcc aaatcttcag gggcctgatc agcttgaatt
      721 ctaataccgt cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagcgaac
      781 gcttccatca gccttcttaa aggagtggtc tttttgatct tgaagaagat ctctgaagat
      841 ccatcggaca cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctggc
      901 ttagcttctt gattgacttc tgagtcctgc agggacccgt gatgatgatg gtgatgaccc
      961 atggtatatc tccttcttaa agttaaacaa aattatttct agagggg
//
