LOCUS       sequence-153562-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma C-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGC
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..371
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggtcatcacc
       61 atcatcatca cgggtccctg caggactcag aagtcaatca agaagctaag ccagaggtca
      121 agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga
      181 tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa
      241 gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg
      301 atcaggcccc tgaagatttg gacatggagg ataacgatat tattgaggct caccgcgaac
      361 agattggagg tgggaagatc accttctatg aggacagggc cttccagggc cgcagctacg
      421 aaaccaccac tgactgcccc aacctgcagc cgtatttcag ccgctgcaac tccatccggg
      481 tggagagcgg ctgctggatg ctctatgagc gtcccaacta ccaaggtcaa caatacttgc
      541 tgcggcgagg ggagtacccc gactaccagc aatggatggg cctcagcgac tccatccgct
      601 cctgttgtct catcccccaa acagtctccc acaggctgcg gctgtacgag agggaagacc
      661 acaaaggcct catgatggag ctgagtgaag actgccccag catccaggac cgcttccacc
      721 tcagcgagat ccgttccctc cacgtgctgg agggctgctg ggtcctctac gagctgccca
      781 actaccgggg gcggcaatac ctgctgaggc cccaagagta caggcggtgc caggactggg
      841 gggccatgga tgctaaggca ggctctttgc ggagagtggt ggatttgtat taatctagag
      901 gatccgaatt cgagctccgt cgacaag
//
