LOCUS       sequence-153563-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma C-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGC
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(626..913)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(926..943)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             956..978
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    993..1017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg agctcgaatt cggatcctct agattaatac aaatccacca
      121 ctctccgcaa agagcctgcc ttagcatcca tggcccccca gtcctggcac cgcctgtact
      181 cttggggcct cagcaggtat tgccgccccc ggtagttggg cagctcgtag aggacccagc
      241 agccctccag cacgtggagg gaacggatct cgctgaggtg gaagcggtcc tggatgctgg
      301 ggcagtcttc actcagctcc atcatgaggc ctttgtggtc ttccctctcg tacagccgca
      361 gcctgtggga gactgtttgg gggatgagac aacaggagcg gatggagtcg ctgaggccca
      421 tccattgctg gtagtcgggg tactcccctc gccgcagcaa gtattgttga ccttggtagt
      481 tgggacgctc atagagcatc cagcagccgc tctccacccg gatggagttg cagcggctga
      541 aatacggctg caggttgggg cagtcagtgg tggtttcgta gctgcggccc tggaaggccc
      601 tgtcctcata gaaggtgatc ttcccacctc caatctgttc gcggtgagcc tcaataatat
      661 cgttatcctc catgtccaaa tcttcagggg cctgatcagc ttgaattcta ataccgtcgt
      721 acaagaatct taaggagtcc atttccttac cctgtctttt agcgaacgct tccatcagcc
      781 ttcttaaagg agtggtcttt ttgatcttga agaagatctc tgaagatcca tcggacacct
      841 ttaaattgat gtgagtctca ggcttgactt ctggcttgac ctctggctta gcttcttgat
      901 tgacttctga gtcctgcagg gacccgtgat gatgatggtg atgacccatg gtatatctcc
      961 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct
     1021 a
//
