LOCUS       sequence-153568-         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma D-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGD
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..365
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggtcat caccatcatc
       61 atcacgggtc cctgcaggac tcagaagtca atcaagaagc taagccagag gtcaagccag
      121 aagtcaagcc tgagactcac atcaatttaa aggtgtccga tggatcttca gagatcttct
      181 tcaagatcaa aaagaccact cctttaagaa ggctgatgga agcgttcgct aaaagacagg
      241 gtaaggaaat ggactcctta agattcttgt acgacggtat tagaattcaa gctgatcagg
      301 cccctgaaga tttggacatg gaggataacg atattattga ggctcaccgc gaacagattg
      361 gaggtgggaa gatcaccctc tacgaggacc ggggcttcca gggccgccac tacgaatgca
      421 gcagcgacca ccccaacctg cagccctact tgagccgctg caactcggcg cgcgtggaca
      481 gcggctgctg gatgctctat gagcagccca actactcggg cctccagtac ttcc
//
