LOCUS       sequence-153569-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human gamma D-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYGD
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(627..914)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(927..944)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             957..979
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattagga gaaatctatg
      121 actctcctca gagagcccac tctggcattc gtggcccccc agtcctggta gcgcctatag
      181 tcccctggca tcagcaggta ctgccgtcct cggtagttgg acagctcgta gaggacccag
      241 gagccctcca gcacgttgag ggagtggatt tcattgaagc ggaagcggtc ctgaagacag
      301 gagcagtcct cagtgaactc tatcatctgg cctctgtagt cctccctctc atagagtctg
      361 atcctgtgag agccagagtg ggggatgagg cggcaggagc ggaccgagtc gctgaggccc
      421 atccactgct ggtggtcggc atagtcgccg cggcgcagga agtactggag gcccgagtag
      481 ttgggctgct catagagcat ccagcagccg ctgtccacgc gcgccgagtt gcagcggctc
      541 aagtagggct gcaggttggg gtggtcgctg ctgcattcgt agtggcggcc ctggaagccc
      601 cggtcctcgt agagggtgat cttcccacct ccaatctgtt cgcggtgagc ctcaataata
      661 tcgttatcct ccatgtccaa atcttcaggg gcctgatcag cttgaattct aataccgtcg
      721 tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc
      781 cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc
      841 tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga
      901 ttgacttctg agtcctgcag ggacccgtga tgatgatggt gatgacccat ggtatatctc
      961 cttcttaaag ttaaacaaaa ttatttctag aggg
//
