LOCUS       sequence-153572-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human alpha A-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYAA
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..371
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggtcatcacc
       61 atcatcatca cgggtccctg caggactcag aagtcaatca agaagctaag ccagaggtca
      121 agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga
      181 tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa
      241 gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg
      301 atcaggcccc tgaagatttg gacatggagg ataacgatat tattgaggct caccgcgaac
      361 agattggagg tatggatgtg accatccagc acccctggtt caagcgcacc ctggggccct
      421 tctaccccag ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc
      481 tgcccttcct gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc
      541 tggactccgg catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg
      601 tgaagcactt ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc
      661 acggaaagca caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc
      721 gctaccgcct gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg
      781 gcatgctgac cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc
      841 gagccatccc cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taatctagag
      901 gatccgaatt cgagctc
//
