LOCUS       sequence-153573-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human alpha A-crystallin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  TITLE     Larry David lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pE-SUMO CRYAA
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(627..914)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(927..944)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             957..979
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc tagattagga cgagggagcc
      121 gaggtgggct tctcctcccg cgacacgggg atggctcgct cggcgtgggt ggcatccagg
      181 ccagtctgga tcttggggcc acagaaggtc agcatgccat cggcagacag ggagcaagag
      241 agggccgact ggtccacgtt ggacggcagg cggtagcggc ggtggaactc acgggaaatg
      301 tagccgtggt cgtcctggcg ctcgttgtgc tttccgtgga tctccacaaa gtcgtcctgc
      361 accttcacgg tgaggtcctc cggggagaag tgcttcacat cgaggaagat gacgaacttg
      421 tcccggtcgg atcgaacctc agagatgccg gagtccagca cggtgcggaa gagggactgg
      481 cggtagtagg ggctgatggt ggacgacagg aagggcagca ggtcatactc aaaaaggccc
      541 tcgccgaaaa actggtcgaa cagccggctg gggtagaagg gccccagggt gcgcttgaac
      601 caggggtgct ggatggtcac atccatacct ccaatctgtt cgcggtgagc ctcaataata
      661 tcgttatcct ccatgtccaa atcttcaggg gcctgatcag cttgaattct aataccgtcg
      721 tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc
      781 cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc
      841 tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga
      901 ttgacttctg agtcctgcag ggacccgtga tgatgatggt gatgacccat ggtatatctc
      961 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatc
//
