LOCUS       sequence-153586-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: scTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP247
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(589..835)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 tgctcaccat gaattcgcgt ttcgaataat tagttgtttt ttgatcttct caagttgtcg
       61 ttaaaagtcg ttaaaatcaa aagcttgtca attggaacca gtcgcaatta tgaaagtaag
      121 ctaataatga tgataaaaaa aaaggtttaa gacagggcag cttccttctg tttatatatt
      181 gctgtcaagt aggggttagg cgcgcccata gggtatcctc actccgattg atgaagagac
      241 ccttctggtt ccaatcctca ctccgattga tgtgacgtca atgggtggag tatatcctca
      301 ctccgattga tgagtgctct cgatgacttc gatatcctca ctccgattga tgaactccgc
      361 cccattgacg caaatcctca ctccgattga tggtggaaca gcccaccctg tacatcctca
      421 ctccgattga tgtgcggcct cgagaagcag ctgaatcctc actccgattg atgactggac
      481 cgtcaccaat tggagatcct cactccgatt gatggtggta aactcgcaat atcctcactc
      541 cgattgatga attgatctgc cgcgatgact tctatatgat atgctagctc tcacttaatc
      601 ttctgtactc tgaagaggag tgggaaatac caagaaaaac atcaaactcg aatgattttc
      661 ccaaacccct accacaagat attcatcagc tgcgagatag gctgatcagg agcaagctcg
      721 tacgagaaga aacaaaatga caaaaaaaat cctatactat ataggttaca aataaaaaag
      781 tatcaaaaat gaagcctgca tctctcaggc aaatggcatt ctgacatcct cttgattaga
      841 atctagcaag accggtctac cgatggtacc tcaagaagcg tagtccggaa cgtcgtacg
//
