LOCUS       sequence-153590-         435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the super-dark state of LOV2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-LOVSD
FEATURES             Location/Qualifiers
     source          1..435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..432
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="ATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRN
                     ARFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQY
                     FIGVQKDGTEHVRDAAEREAVMEIKKTAEEIDEAAKEL"
ORIGIN
        1 cccggggcta ctacacttga acgtattgag aagaactttg tcattactga cccaagattg
       61 ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga atatagccgt
      121 gaagaaattt tgggaagaaa cgccaggttt ctacaaggtc ctgaaactga tcgcgcgaca
      181 gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca gctgattaat
      241 tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat gcgagatcag
      301 aagggagatg tccagtactt tattggggtt cagaaggatg gaactgagca tgtccgagat
      361 gctgccgaga gagaggcagt catggagatt aagaaaactg cagaagaaat tgatgaggcg
      421 gcaaaagaac tttaa
//
