LOCUS       sequence-153592-         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of LOV I427T, a mutant with super short 
            relaxation time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-LOV2 I427T
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..429
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPTIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 ttggctacta cacttgaacg tattgagaag aactttgtca ttactgaccc aagattgcca
       61 gataatccca ctatattcgc gtccgatagt ttcttgcagt tgacagaata tagccgtgaa
      121 gaaattttgg gaagaaactg caggtttcta caaggtcctg aaactgatcg cgcgacagtg
      181 agaaaaatta gagatgccat agataaccaa acagaggtca ctgttcagct gattaattat
      241 acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc agcctatgcg agatcagaag
      301 ggagatgtcc agtactttat tggggttcag ttggatggaa ctgagcatgt ccgagatgct
      361 gccgagagag agggagtcat gctgattaag aaaactgcag aaaatattga tgaggcggca
      421 aaagaacttt aa
//
