LOCUS       sequence-153598-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Coseno M, Martin G, Berger C, Gilmartin G, Keller W, Doublie S
  TITLE     Crystal structure of the 25 kDa subunit of human cleavage factor Im.
  JOURNAL   Nucleic Acids Res. 2008 Jun;36(10):3474-83. doi: 10.1093/nar/gkn079.
            Epub 2008 Apr 29.
  PUBMED    18445629
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn079"; journalName: "Nucleic Acids Res"; date: 
            "2008-06"; volume: "36"; issue: "10"; pages: "3474-83"
COMMENT     Sequence Label: HisMBP CFlm25
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(158..163,164..179,180..186)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     199..221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             289..306
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
       61 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
      121 atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc atcggctcgt
      181 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcca gtccgtttag
      241 gtgttttcac gagcacttca ccaacaagga ccatagatta tgaaaatcca tcaccatcac
      301 catcacgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
      361 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
      421 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
      481 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
      541 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
      601 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
      661 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
      721 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
      781 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
      841 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
      901 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt t
//
