LOCUS       sequence-153602-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing his-MBP tagged AsCpf1 (E.coli codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Hur JK, Kim K, Been KW, Baek G, Ye S, Hur JW, Ryu SM, Lee YS, Kim JS
  TITLE     Targeted mutagenesis in mice by electroporation of Cpf1 
            ribonucleoproteins.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596.
  PUBMED    27272385
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596."
COMMENT     Sequence Label: pDEST-hisMBP-AsCpf1-EC
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(157..162,163..178,179..185)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    193..209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     198..220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             288..305
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
       61 tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
      121 tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca tcggctcgta
      181 taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagccag tccgtttagg
      241 tgttttcacg agcacttcac caacaaggac catagattat gaaaatccat caccatcacc
      301 atcacgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
      361 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
      421 ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct
      481 tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
      541 cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg
      601 gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc
      661 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
      721 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
      781 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
      841 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
      901 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
      961 aaacagcgat gaccatcaac
//
